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Nucleotide and amino acid sequences of a 2,942-bp fragment which contains most of the gyrB gene and the 5′ end of the gyrA gene of the C. trachomatis L2 reference strain. Specific primers are indicated by arrows. +, nucleotide mutation (G→T) in the gyrA gene leading to the Ser→Ile substitution in the GyrA protein. Tsp509I restriction sites (AATT) are underlined; the site indicated in italics corresponds to the site created by the mutation (G→T).
