Figure 6.

rab-5 D135N heterozygotes are defective in endocytic uptake. (A) Confocal images showing the steady-state ssGFP level in coelomocytes (yellow outline) of a rab-5 D135D#1 control edit heterozygote (top) and a D135N#2 variant edit heterozygote (bottom). Scale bar, 5 μm. All genotypes were generated by crosses with wild-type males; the full genotypes of the parental strains are listed in Supplementary Material, Table S2. (B) Quantification of the mean GFP fluorescent intensity from rab-5 D135D#1 (circles) (n = 17) and D135N#2 (triangles) (n = 18) heterozygous coelomocytes. The scatter plot shows the mean and standard deviation. Differences between groups were determined by Student’s t-test. ^****P < 0.0001.