Table 5.
EC50 values [μM] of AAP analogs against 7 different mammalian cell lines. A Melanoma; B Colon cancer; C breast cancer; D Ovarian cancer; E Mouse fibroblasts; F Cervical cancer; G Human embryonic kidney cancer. The assay was performed in triplicates. Standard deviations are displayed if applicable-
| A375A | HT29B | MCF7C | A2780D | NIH 2T3E | HeLaF | HEK293G | |
|---|---|---|---|---|---|---|---|
| MMV845 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 6 | > 30 | > 30 | 16.4 ± 1.7 | 19.8 ± 2.0 | > 30 | > 30 | 19.1 ± 2.4 |
| 7 | 18.6 ± 4.2 | > 30 | 13.6 ± 1.4 | 14.9 ± 1.6 | > 30 | > 30 | 6.3 ± 1.2 |
| 11 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 12 | 29.4 ± 1.4 | > 30 | > 30 | 28.3 ± 1.4 | 28.5 ± 2.0 | 28.3 ± 1.5 | n.d. |
| 14 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 |
| 15 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 |
| 17 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 18 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 20 | > 30 | > 30 | > 30 | 23.6 ± 2.2 | > 30 | > 30 | > 30 |
| 24 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 28 | > 30 | > 30 | 23.2 ± 2.8 | 24.0 ± 5.2 | 26.9 ± 5.0 | > 30 | > 30 |
| 29 | > 30 | > 30 | 25.9 ± 2.7 | 23.2 ± 4.4 | 24.2 ± 4.2 | > 30 | > 30 |
| 32 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 34 | > 30 | > 30 | > 30 | > 30 | > 30 | 29.8 ± 1.3 | n.d. |
| 36 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 37 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 39 | > 30 | 29.8 ± 1.7 | > 30 | 29.7 ± 1.2 | > 30 | > 30 | n.d. |
| 40 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 |
| 42 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 43 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 45 | > 30 | 29.8 ± 1.9 | > 30 | 29.5 ± 2.0 | 29.0 ± 1.3 | > 30 | n.d. |
| 46 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 50 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 51 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 53 | > 30 | > 30 | > 30 | > 30 | > 30 | > 30 | n.d. |
| 54 | 29.3 ± 1.5 | > 30 | 28.9 ± 1.8 | 29.0 ± 1.7 | > 30 | > 30 | n.d. |