FIG. 6.

Effect of AY on the in vitro production of IL-12 (A), the synthesis of gamma interferon (B), and the IL-12 mRNA level (C). PBMCs from patients with AIDS were cultured for 15 days in complete medium in the presence (+AY) or in the absence (0) of 3 × 10⁻⁶ M AY. Cytokine production (A and B) was measured in cell-free supernatants with an R&D system enzyme-linked immunosorbent assay kit (the results are means ± SDs of three experimental values). The levels of IL-12 and GAPDH mRNA expression (C) were studied in PBMCs from four HIV-1-infected (HIV⁺) symptomatic individuals (subjects to M1 to M4; CD4⁺-cells-counts, <200/mm³) as described in Materials and Methods.