TABLE 1.

Effect of AY on HIV-1 production and cell mortality in PBMCs from patients with AIDS^a

Time (days)	Donor	Production of HIV-1 in culture supernatants				% Mortality of cultured cells
		p24 protein (pg/ml)		HIV-1 particles (counts/ml of medium)
		AY−	AY+	AY−	AY+	AY−	AY+
10	M1	5	4	900	700	23	9
14	M1	10	9	800	770	28	8
22	M1	700	6	5,170	822	26	10
10	M2	−1	2	650	725	18	10
14	M2	3	5	921	680	55	11
22	M2	2,500	25	7,500	1,000	70	12
10	M3	10	7	1,100	652	16	12
14	M3	1,800	2,200	2,541	3,161	13	12
22	M3	15	10	650	811	30	13
10	M4	5	4	750	1,000	18	7
14	M4	7	10	1,000	1,400	25	10
22	M4	200	150	1,300	1,000	28	13
10	M5	5	4	400	500	25	10
14	M5	10	6	700	641	32	11
22	M5	8	−2	500	400	35	12
10	M6	−1	3	600	400	19	9
14	M6	2	1	500	520	29	8
22	M6	4	3	420	380	40	13
10	M7	4	7	619	542	20	13
14	M7	6	5	531	600	27	12
22	M7	−1	2	450	550	31	14
10	M8	−1	3	600	575	25	11
14	M8	2	4	500	450	35	14
22	M8	4	3	480	530	42	16

^aAfter activation with PHA, PBMCs were cultured for up to 25 to 30 days in complete medium with recombinant IL-2 in the presence (AY+) or in the absence (AY−) of the molecule. The cells were harvested for cell viability determination, and the culture medium was replaced every 3 days in order to maintain a concentration of 8 × 10⁵ cells/ml. The culture supernatants were used to test both HIV-1 p24 antigen levels by enzyme-linked immunosorbent assay and reverse transcriptase activity. Mortality was assessed by blue Trypan blue coloration. Data are expressed as the means for triplicate cultures. The standard deviation was less than 10% of the mean.