TABLE 2.

Effect of AY on gamma interferon and IL-12 production and cell mortality in PBMCs from patients with AIDS^a

Time (days)	Donor	Cytokine production in culture supernatants				% Mortality of cultured cells
		Gamma interferon (pg/ml)		IL-12 (pg/ml)
		AY−	AY+	AY−	AY+	AY−	AY+
10	M1	20	200	5	25	23	9
14	M1	15	250	5	20	28	8
22	M1	10	300	7	30	26	10
10	M2	10	40	4	18	18	10
14	M2	20	50	5	20	55	11
22	M2	15	70	7	25	70	12
10	M3	10	350	5	30	16	12
14	M3	10	300	5	25	13	12
22	M3	10	400	5	40	30	13
10	M4	20	750	10	50	18	7
14	M4	15	600	15	60	25	10
22	M4	20	850	20	80	28	13
10	M5	20	600	10	100	25	10
14	M5	15	500	5	90	32	11
22	M5	10	800	5	110	35	12
10	M6	15	200	15	150	19	9
14	M6	20	250	10	100	29	8
22	M6	10	300	5	100	40	13
10	M7	10	150	20	100	20	13
14	M7	15	180	30	150	27	12
22	M7	20	250	25	120	31	14
10	M8	15	350	15	90	25	11
14	M8	20	400	20	80	35	14
22	M8	25	450	25	95	42	16

^aThe cells were cultured as described in footnote a of Table 1. The culture supernatants were used to test both gamma interferon and IL-12 levels with an R&D system enzyme-linked immunosorbent assay kit. Mortality was assessed by Trypan blue coloration. Data are expressed as the means for triplicate cultures.