TABLE 3.

Dissociation kinetics of [³H]VU6013720 induced by multiple ligands

Dissociation was performed by adding 10 μM of atropine, VU6013719, or VU0467154 at designated times. Data were fit with both one- and two-phase decay curves. For atropine and VU6013719, two-site fits were the preferred model; for VU0467154, a one-site model was preferred. *P < 0.05, **P < 0.01, ***P < 0.001 between atropine and VU6013719.

	% Fast	Koff fast (min−1)	Koff slow (min−1)	Half-life fast (min)	Half-life slow (min)	Plateau	R2
Atropine	51 ± 5	0.56 ± 0.39	0.05 ± 0.01	1.6 ± 0.9	15 ± 3	1.0 ± 0.7	0.98 ± 0.02
VU6013719	44 ± 8	2.0 ± 0.5*	0.17 ± 0.02***	0.40 ± 0.10	4.2 ± 0.5**	−2.2 ± 3.2	0.98 ± 0.01

		Koff (min−1)		Half-life (min)		Plateau	R2
VU0467154		0.60 ± 0.18		1.2 ± 0.3		58 ± 5	0.84 ± 0.05

K_off, dissociation off rate.