TABLE 1.

Efficacy and potency values of indicated 5-HT1AR agonists acting on each Gα_i/o/z protein. Each value represents the mean ± S.D. of 5–10 independent replicates

Compound	Gα protein	Emax ± S.D.	pEC50 ± S.D.	Compound	Gα protein	Emax ± S.D.	pEC50 ± S.D.
	GoA	0.082 ± 0.003	7.20 ± 0.15		GoA	0.048 ± 0.010	4.78 ± 0.18
	GoB	0.066 ± 0.015	6.59 ± 0.46		GoB	0.040 ± 0.007	4.82 ± 0.39
5-HT	Gi1	0.045 ± 0.002	6.52 ± 0.07	Nuciferine	Gi1	0.030 ± 0.003
	Gi2	0.060 ± 0.007	6.86 ± 0.09		Gi2	0.013 ± 0.009	4.75 ± 1.15
	Gi3	0.042 ± 0.005	6.78 ± 0.06		Gi3	0.014 ± 0.009
	Gz	0.061 ± 0.006	6.36 ± 0.06		Gz	0.020 ± 0.002	4.90 ± 0.66
	GoA	0.084 ± 0.020	7.33 ± 0.20		GoA	0.036 ± 0.006	5.17 ± 0.20
	GoB	0.070 ± 0.008	7.14 ± 0.06		GoB	0.042 ± 0.006	4.72 ± 0.18
8-OH-DPAT	Gi1	0.032 ± 0.002	6.68 ± 0.18	Sumatriptan	Gi1	0.021 ± 0.004
	Gi2	0.054 ± 0.016	7.05 ± 0.18		Gi2	0.018 ± 0.005	4.92 ± 0.63
	Gi3	0.032 ± 0.003	6.77 ± 0.12		Gi3	0.013 ± 0.007
	Gz	0.053 ± 0.015	6.70 ± 0.19		Gz	0.036 ± 0.004	4.81 ± 0.08
	GoA	0.050 ± 0.006	6.57 ± 0.10		GoA	0.106 ± 0.019	7.88 ± 0.15
	GoB	0.025 ± 0.004	6.97 ± 0.09		GoB	0.071 ± 0.010	8.13 ± 0.14
Buspirone	Gi1	0.011 ± 0.003	7.20 ± 0.20	Vilazodone	Gi1	0.043 ± 0.004	6.97 ± 0.08
	Gi2	0.017 ± 0.005	7.33 ± 0.19		Gi2	0.045 ± 0.004	5.75 ± 0.12
	Gi3	0.012 ± 0.007	6.66 ± 0.87		Gi3	0.039 ± 0.006	7.34 ± 0.19
	Gz	0.024 ± 0.006	6.85 ± 0.18		Gz	0.061 ± 0.005	5.81 ± 0.13
	GoA	0.032 ± 0.009	5.88 ± 0.21		GoA	0.053 ± 0.018	4.94 ± 0.18
	GoB	0.020 ± 0.005	5.76 ± 0.28		GoB	0.050 ± 0.010	5.02 ± 0.22
Aripiprazole	Gi1			Vortioxetine	Gi1	0.049 ± 0.009	4.86 ± 0.14
	Gi2	0.011 ± 0.003	4.03 ± 0.82		Gi2	0.037 ± 0.008	4.98 ± 0.30
	Gi3	0.013 ± 0.001			Gi3	0.031 ± 0.013	5.08 ± 1.02
	Gz	0.020 ± 0.002			Gz	0.048 ± 0.009	5.14 ± 0.20
	GoA	0.078 ± 0.019	6.77 ± 0.14		GoA	0.077 ± 0.014	6.27 ± 0.11
	GoB	0.097 ± 0.008	6.93 ± 0.12		GoB	0.081 ± 0.017	7.77 ± 0.21
Flibanserin	Gi1	0.061 ± 0.003	5.96 ± 0.38	F-15599	Gi1	0.060 ± 0.008	7.24 ± 0.14
	Gi2	0.054 ± 0.013	6.13 ± 0.23		Gi2	0.066 ± 0.003	5.71 ± 0.10
	Gi3	0.059 ± 0.010	6.08 ± 0.19		Gi3	0.056 ± 0.002	7.76 ± 0.11
	Gz	0.070 ± 0.008	5.91 ± 0.32		Gz	0.060 ± 0.004	5.67 ± 0.13