Figure 1.

Defining the layers in the mPFC by using antibodies developed against Calb and transcription factors WFS1, Ctip2, and FoxP2. A, A low-magnification multicolor confocal image taken from an mPFC section immunostained against WFS1, Calb, Ctip2, and FoxP2. White empty rectangles indicate the PrL subregion of the mPFC and the S1 (shown at a higher magnification in B–E). Scale bar, 1 mm. B–E, Higher-magnification confocal images taken from the S1 and PrL region and immunolabeled for WFS1, Calb, Ctip2, and FoxP2. Dashed white lines indicate the boundaries of the layers (L) defined by the immunolabeling. F, A multicolor confocal image taken from a PrL region that was immunostained against WFS1 (green), Ctip2 (yellow), and FoxP2 (magenta), indicating the clear boundaries of the layers. G, H, Immunostaining against PV and CB1 in the PrL region of mPFC. Scale bar, 100 µm.