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. 2023 Oct 18;43(42):6972–6987. doi: 10.1523/JNEUROSCI.0432-23.2023

Figure 5.

Figure 5.

Distribution of dendritic and axonal arbor of different basket cell types within the layers of the mPFC. A1–2, Cluster analysis of CCKBCs determined the superficial and deep categories based on the location of their dendrites, and analysis of PVBCs divided them into three subgroups based on the location of their dendrites and axons. B1–5, Neurolucida reconstructions of dendritic and axonal arbor of different basket cells labeled in slice preparations. Black lines represent the axons, and colored lines show the dendritic trees, respectively. The dendrites of superficial CCKBCs are shown in light blue (B1; n = 7 from 4 mice), and the dendrites of deep CCKBCs are shown in dark blue (B2; n = 8 from 6 mice). The dendrites of layer (L)2/3 PVBCs are shown in yellow (B3; n = 9 from 9 mice), the dendrites of L5a PVBCs are shown in light orange (B4; n = 14 from 10 mice), and the dendrites of L5b PVBCs are shown in bright orange (B5; n = 9 from 7 mice). Dashed dark gray lines represent the boundaries of the layers. Scale bar, 100 µm. C1–5, The distribution of dendritic lengths in the different layers. D1–5, The distribution of axonal lengths in the different layers. E, Comparison of the distribution of the dendritic lengths in different layers for the two CCKBC subgroups (M–W tests by layers, L1, p = 0.001; L2/3, p = 0.013; L5a, p = 0.001; L5b, p = 0.016). F, Comparison of the distribution of the axonal lengths of the two CCKBC subgroups in different layers (M–W tests by layers, L1, p = 0.013; L2/3, p = 0.003; L6, p = 0.019). G, Comparison of the distribution of dendritic lengths of the three PVBCs subgroups in different layers (K–W ANOVA by layers, L1, p < 0.001; L2/3, p < 0.001; L5a, p < 0.001; L5b, p < 0.001; L6, p = 0.007; post hoc Dunn's test by layers, L1, L2/3 PVBC vs L5a PVBC, p = 0.002; L2/3 PVBC vs L5b PVBC, p < 0.001; L5a PVBC vs L5b PVBC, n.s.; L2/3, L2/3 PVBC vs L5a PVBC, p = 0.005; L2/3 PVBC vs L5b PVBC, p < 0.001; L5a PVBC vs L5b PVBC, n.s.; L5a, L2/3 PVBC vs L5a PVBC, p = 0.006; L2/3 PVBC vs L5b PVBC, n.s.; L5a PVBC vs L5b PVBC, p < 0.001; L5b, L2/3 PVBC vs L5a PVBC, p = 0.03; L2/3 PVBC vs L5b PVBC, p < 0.001; L5a PVBC vs L5b PVBC, p = 0.008; L6, L2/3 PVBC vs L5a PVBC, n.s.; L2/3 PVBC vs L5b PVBC, p = 0.005; L5a PVBC vs L5b PVBC, n.s.). H, Comparison of the distribution of axons of the three PVBCs subgroups in different layers. (K–W ANOVA by layers, L1, p < 0.001; L2/3, p < 0.001; L5a, p < 0.001; L5b, p < 0.001; L6, p = 0.005; post hoc Dunn's test by layers, L1, L2/3 PVBC vs L5a PVBC, p = 0.005; L2/3 PVBC vs L5b PVBC, p < 0.001; L5a PVBC vs L5b PVBC, n.s.; L2/3, L2/3 PVBC vs L5a PVBC, p = 0.007; L2/3 PVBC vs L5b PVBC, p < 0.001; L5a PVBC vs L5b PVBC, p = 0.04; L5a, L2/3 PVBC vs L5a PVBC, p < 0.001; L2/3 PVBC vs L5b PVBC, n.s.; L5a PVBC vs L5b PVBC, p < 0.001; L5b, L2/3 PVBC vs L5a PVBC, p = 0.02; L2/3 PVBC vs L5b PVBC, p < 0.001; L5a PVBC vs L5b PVBC, p = 0.01; L6, L2/3 PVBC vs L5a PVBC, n.s.; L2/3 PVBC vs L5b PVBC, p = 0.004; L5a PVBC vs L5b PVBC, n.s.).