Fig 2. Simulation of virus infection and replication within a single mosquito.

A: Numbers of infected cells over time. B: Numbers of virus particles over time. The model tracks numbers of virions in the blood meal (G_v), numbers of infected midgut cells (M_c) (total cells 400 (c_max)), numbers of virions produced in the midgut (M_v), numbers of infected hemocoel cells (H_c) (total cells available 900 (h_max)) and numbers of virions in the hemocoel (H_v). The model is run in hourly time steps and a single simulation was run for the equivalent of seven days, with an initial input of 3000 virus particles (G_v). The rate at which susceptible cells become infected (β) was set to 10⁻⁸ h⁻¹, the virus decay rate (μ_V) was 0.1 h⁻¹, the rate virions spread between cells (α) was 10^−3.5 h⁻¹, the rate at which virions are produced by infected cells (γ) was 10 h⁻¹ and the rate virions escape from the midgut into the hemocoel (ρ) was 0.05 h⁻¹.