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. 2023 Oct 19;21(10):e3002336. doi: 10.1371/journal.pbio.3002336

Fig 3. TFs and TF hierarchy controlling distinct epithelial cell identity.

Fig 3

(A) Heatmap of motif enrichment Z-scores detected in variable CREs and the corresponding TFs. The percentage of CREs containing the motifs and the expression ratio of TFs in LSCs and KCs are indicated. (B) ANANSE influence score plot of TFs identified in ESC-KC (x-axis) and ESC-LSC (y-axis) comparison. Circle size represents the maximum number of target genes of a TF. The color represents log2FC between LSC/KC (orange LSC high; purple KC high). (C) ANANSE influence score plot of TFs identified in KC-LSC comparison. (D) ANANSE influence score plot of TFs identified in LSC-KC comparison. (E) TF hierarchy is indicated by the binding score of a TF to its target TF locus, and the cell type-specific regulation is indicated by the binding score difference of the TF at the target TF locus between cell types. When a binding score difference in KC-LSC comparison is greater than the mean of the difference in ESC-KC and ESC-LSC comparison, this TF regulation of the target TF is annotated as either KC- (purple arrows) or LSC-specific (orange arrows) regulation. Otherwise, the regulation is annotated as “shared regulation” for both cell types (gray arrows). The degree of binding score difference is indicated by the thickness of the arrows. Outdegree node size represents the number of target genes. Fold change of TF gene expression in LSC and KCs is represented by orange (LSC-high) and purple (KC-high) colors. For the underlying data, see the Zenodo entry [51]. CRE, cis-regulatory element; ESC, embryonic stem cell; KC, keratinocyte; LSC, limbal stem cell; TF, transcription factor.