Figure 2.

Substrate Scope

a. Reaction conditions: amide (20 μmol), purified GluER enzyme (1 mol%, 200 nmol), 100 mM buffer (18 mM final substrate concentration), iPrOH (10 % v/v), glucose (40 μmol), GDH-105 (10 wt%, 0.4–7.5 mg/rxn), NADP⁺ (2 mol%), 48 hours, 25 °C. Yields were determined by NMR analysis using trimethoxy benzene as an internal standard. See Supplemental Information for detailed experimental procedure and additional optimization studies.