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Intermolecular Allylation
a. Reaction conditions: 18 (20 μmol, 2.06μL), 19–21 (80 μmol) purified GluER enzyme (1 mol%, 200 nmol), 100 mM buffer (18 mM final substrate concentration), iPrOH (10 % v/v), glucose (40 μmol), GDH-105 (10 wt%, 0.4–7.5 mg/rxn), NADP+ (2 mol%), 48 hours, 25 °C. Yields were determined by HPLC using calibration curves. See Supplemental Information for detailed experimental procedure and additional optimization studies.
