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. Author manuscript; available in PMC: 2023 Oct 19.
Published in final edited form as: Cold Spring Harb Protoc. 2023 Oct 3;2023(10):pdb.prot108185. doi: 10.1101/pdb.prot108185
Step Time Temperature Cycles
Initial denaturation 1 min 95°C 1
Denaturation 30 sec 95°C 20
Annealing 30 sec 52°C
Polymerization 2 min 72°C
Extra extension 2 min 72°C 1
Hold 10°C 1