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. Author manuscript; available in PMC: 2023 Oct 19.
Published in final edited form as: Cold Spring Harb Protoc. 2023 Oct 3;2023(10):pdb.prot108185. doi: 10.1101/pdb.prot108185
Reagent Quantity for 10 mL
Orange G (Fisher Scientific AC416550100) 100 mg
Tris-EDTA solution (10×, pH 7.4; Fisher Scientific BP2477100) 5 mL
Glycerol (80% v/v) 5 mL
1. Add Orange G to 10× Tris-EDTA solution (pH 7.4).
2. Vortex to dissolve.
3. Add 80% glycerol.
4. Vortex to mix.
5. Store at room temperature.