Table 3. Overview of libraries used for comparisons in each biological contrast.

PBMC - Peripheral Blood Monocyte Cells, RRA - Robust Rank Aggregation, MLE - Maximum Likelihood Estimation.

Biological contrast	Control	Treatment	CRISPR-screen hits identification method	FDR cutoff [%]
Tumor screen	Plasmid gRNAs libraries	Only tumor cells without PBMC	RRA	2
Antigen-independent tumor killing	Only tumor cells without PBMC	Tumor cells not loaded with antigen with PBMC	MLE	5
Antigen-dependent tumor killing	Tumor cells not loaded with antigen with PBMC	Tumor cells loaded with antigen with PBMC	MLE	5