Table 4. General design matrix for MLE comparison for specificity of antigen in- and dependent CRISPR-Cas9 screens.

TC - tumor cells; PBMC - co-culture with PBMC or lack of it (noPBMC), AG - PBMC antigen stimulation or lack of it (noAG); rep1,2,3 – technical replicates.

Samples	Baseline	Antigen independent	Antigen dependent
TC_noPBMC_noAG_rep1	1	0	0
TC_noPBMC_noAG_rep2	1	0	0
TC_noPBMC_noAG_rep3	1	0	0
TC_PBMC_noAG_rep1	1	1	0
TC_PBMC_noAG_rep2	1	1	0
TC_PBMC_noAG_rep3	1	1	0
TC_PBMC_AG_rep1	1	0	1
TC_PBMC_AG_rep2	1	0	1
TC_PBMC_AG_rep3	1	0	1