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Formation of phosphate esters of nucleoside analogs in virus-infected MRC-5 cells. MRC-5 cells infected with HSV-1 (1), HSV-2 (2), or VZV (3) were treated with [3H]A-5021 (a), [3H]ACV (b), or [3H]PCV (c) at the indicated concentrations. At the designated times, cells were extracted, and the extracts were analyzed by HPLC. Open circles, triphosphate; closed triangles, diphosphate; closed squares, monophosphate.
