FIG. 7.

Chain termination by A-5021 triphosphate (A-5021TP) or ACV triphosphate (ACVTP). HSV-2 DNA polymerase was reacted with M13mp18 single-stranded DNA and specific oligomer (17 mer) as a template and a primer, respectively, in the presence of fixed concentrations of natural dNTPs and various concentrations of A-5021TP or ACVTP (lane 1, 1 mM; lane 2, 300 μM; lane 3, 100 μM; lane 4, 30 μM; lane 5, 10 μM; lane 6, 3 μM; lane 7, 1 μM). Cont., no chain terminator. Lanes ddG, ddA, ddT, and ddC, dideoxy sequencing with ddGTP, ddATP, ddTTP, and ddCTP, respectively. Samples were analyzed on a 6% sequencing gel, followed by autoradiography.