FIG. 8.

DNA chain extension by HSV-2 DNA polymerase. The reaction was performed with M13mp18 single-stranded DNA and a 5′ ³²P-end-labeled specific oligomer (17 mer) as the template and the primer, respectively, in the presence or absence of 50 μM dATP, dTTP, and/or inhibitor as shown. Lanes ddG, ddA, ddT, and ddC, dideoxy sequencing with ddGTP, ddATP, ddTTP, and ddCTP, respectively. Samples were analyzed on a 15% sequencing gel, followed by autoradiography.