Fig. 7. PVN^MC4R inhibition is required for conterregulatory eating.

a Schematic of NE/E measurements from PVN^MC4R neurons. b, c Average fiber photometry trace (b) and individual heat maps (c) showing GRAB_NE2h signal in response to 2DG injection. d Summary graph for quantification of GRAB_NE2h fluorescence in PVN^MC4R neurons (n = 4 mice, two-tailed, paired t-test, *p = 0.042). e Schematic for monitoring PVN^MC4R activity in vivo with GCaMP7s. f–h Effect of intraperitoneal 2DG administration on PVN^MC4R GCaMP7s fluorescence (n = 5 mice). f Average traces, g individual temporal heatmaps, and h quantification of mean z-score from 10–60 min (two-tailed, paired t-test, *p = 0.032). i Schematic for chemogenetic PVN^MC4R activation with hM3Dq. j Representative image of hM3Dq expression in PVN^MC4R neurons (scale bar: 50 μm). k Effect of PVN^MC4R activation on 2DG-induced feeding in control (n = 6 mice) and hM3Dq expressing (n = 6 mice) animals (2-way RM ANOVA with Tukey’s multiple comparison, ***p < 0.009, n.s.: Not significant). Data are presented as mean values +/− SEM.