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. 2023 Oct 19;13:17841. doi: 10.1038/s41598-023-45220-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The concentration-dependent effect of Aβ on inflammasome protein and proinflammatory cytokine expression in SH-SY5Y cells. Cells were incubated with various concentration (01, 1 and 2 µM) of Aβ for 24 min. Western blot analysis was used to determine the expression levels of (a) NLRP3, (b) ASC, (c) Caspase 1, (d) pro-IL-1β, (e) IL-1β, (f) pro-IL-18, (g) IL-18 and (h) TNF-α. The band densities were normalized to actin. The ratios were calculated as a percentage of the respective value in the control group. The data are expressed as the means ± S.E.M. One-way ANOVA and Tukey's pos-hoc test were performed for statistical analysis. N = 3–4 (*, **, ***, ****denote statistical significance at p < 0.05, p < 0.01, p < 0.001 and p < 0.0001 compared to the control group, respectively).