Figure 1.

EGR-1 expression of Opn5 KO 661w cells was not upregulated by violet light (VL) exposure. (A) The time points for the collection of total RNA and protein from 661w cells for western blot and real-time PCR. Cells were exposed to 280 µW/cm² of VL (360–400 nm) for 2 h and subsequently cultured in the dark. At designated time points, cells were collected for western blotting or real-time PCR analyses. (B) Egr-1 mRNA expression level of Opn5 KO 661w cells before and after VL exposure (n = 6 per group). (C) A representative image and quantitative analysis of western blot of EGR-1 expression (n = 4 per group). The membrane images were cropped to remove irrelevant parts (Supplementary Fig. S2). Bars represent mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA with a post hoc Tukey test.