TABLE 1.
EVs’ size, quantitation, and protein concentration in the oviductal fluid (oEVs) and uterine fluid (uEVs) from artificially inseminated sows with three different types of accumulative fractions of the boar ejaculate: AI-F1 (n = 5), AI-F2 (n = 5), and AI-F3 (n = 5); and from non-inseminated sows: C (n = 5). F1, sperm-rich fraction (SRF); F2, F1 plus the intermediate fraction; F3, F2 plus the post-SRF.
AI-F1 | AI-F2 | AI-F3 | C | ||
---|---|---|---|---|---|
Size (nm) | oEVs | 297.76 ± 89.45 | 262.60 ± 98.57 | 234.84 ± 5.67 | 215.52 ± 9.65 |
uEVs | 142.40 ± 30.53 | 202.92 ± 18.20 | 176.86 ± 8.45 | 163.86 ± 12.45 | |
[EVs] (x 109 particles/mL) | oEVs | 278.36 ± 10.32 | 392.28 ± 20.79 | 404.07 ± 26.29 | 414.79 ± 106.53 |
uEVs | 321.92 ± 8.05a | 395.14 ± 32.00a | 525.14 ± 32.81ab | 687.28 ± 85.19b | |
[Protein] (µg/µL) | oEVs | 0.51 ± 0.07 | 0.55 ± 0.05 | 0.53 ± 0.06 | 0.58 ± 0.05 |
uEVs | 0.86 ± 0.06a | 0.86 ± 0.04a | 1.19 ± 0.09b | 1.30 ± 0.06b |
Data are represented as the mean ± standard error of the mean (SEM). Different superscripts (a,b) within the same row indicate statistical differences between experimental groups (p < 0.05).