TABLE 3.
Susceptibility patterns of S. aureus gyrB+ strains diploid for grlA
| Strain/plasmid |
grlAa
|
MIC (μg/ml)b
|
||||
|---|---|---|---|---|---|---|
| Chromosome | Plasmid | Ciprofloxacin | Norfloxacin | Novobiocin | Coumermycin | |
| RN4220/pSK950 | Wild | None | 0.5 | 1.0 | 0.16 | 0.02 |
| RN4220/pBFMTA | Wild | Ala116Glu | 1.0 | 4.0 | 0.08 | 0.01 |
| RN4220/pBFC2A | Wild | Ala116Pro | 1.0 | 4.0 | 0.04 | 0.01 |
| RN4220/pBFC4A | Wild | Ser80Phe | 1.0 | 8.0 | 0.16 | 0.02 |
| BF6/pSK950 | Ala116Glu | None | 1.0 | 8.0 | 0.04 | 0.01 |
| BF6/pBFISA | Ala116Glu | Wild | 0.25 | 1.0 | 0.08 | 0.01 |
| BF5/pSK950 | Ala116Pro | None | 1.0 | 8.0 | 0.04 | 0.01 |
| BF5/pBFISA | Ala116Pro | Wild | 0.25 | 1.0 | 0.08 | 0.01 |
| EN20/pSK950 | Ser80Phe | None | 2.0 | 16.0 | 0.16 | 0.02 |
| EN20/pBFISA | Ser80Phe | Wild | 0.25 | 1.0 | 0.16 | 0.01 |
a
Wild, wild-type gene; none, plasmid vector only.
b
The MICs are the mean values for at least three different transformants.