TABLE 4.
Susceptibility patterns of S. aureus RN4220 gyrB142 strains diploid for grlAa
| Strain/plasmid |
grlAb
|
MIC (μg/ml)c
|
||||
|---|---|---|---|---|---|---|
| Chromosome | Plasmid | Ciprofloxacin | Norfloxacin | Novobiocin | Coumermycin | |
| BF2/pSK950 | Wild | None | 0.5 | 1.0 | 20.0 | 5.0 |
| BF2/pBFMTA | Wild | Ala116Glu | 1.0 | 4.0 | 5.0 | 1.25 |
| BF2/pBFC2A | Wild | Ala116Pro | 1.0 | 4.0 | 5.0 | 1.25 |
| BF2/pBFC4A | Wild | Ser80Phe | 1.0 | 4.0 | 10.0 | 2.5 |
| BF7/pSK950 | Ala116Glu | None | 1.0 | 8.0 | 5.0 | 1.25 |
| BF7/pBFISA | Ala116Glu | Wild | 0.25 | 0.5 | 20.0 | 5.0 |
| BF8/pSK950 | Ala116Pro | None | 1.0 | 4.0 | 5.0 | 1.25 |
| BF8/pBFISA | Ala116Pro | Wild | 0.25 | 0.5 | 20.0 | 5.0 |
| BF9/pSK950 | Ser80Phe | None | 2.0 | 8.0 | 20.0 | 5.0 |
| BF9/pBFISA | Ser80Phe | Wild | 0.25 | 0.5 | 20.0 | 5.0 |
a
BF2, BF7, BF8, and BF9 carry the double-mutation (Ser102 and Ile144) gyrB gene.
b
Wild, wild-type gene; none, plasmid vector only.
c
The MICs are the mean values for at least three different transformants.