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. 2023 Sep 22;26(11):107996. doi: 10.1016/j.isci.2023.107996

Figure 1.

Figure 1

AG/LEAP-2 ratio is a sensor of chronic food restriction and refeeding in female mice

Food-restricted mice of both FR and FRW group exhibited significant weight loss compared to ad libitum fed controls (A) but progressive refeeding over 10 days restored their body weight. Chronic food restriction was associated with increased plasma levels of acyl-ghrelin (B) and decreased levels of LEAP-2 (C) independently of running wheel in home-cage. AG/LEAP-2 ratio was higher in FR and FRW mice compared to ad libitum fed controls with no effect of the running wheel. Progressive refeeding lead to a significant decrease in AG plasma levels (F) and increase in LEAP-2 levels (G) leading to decreased AG/LEAP-2 molar ratio in refed FR and FRW mice. n = 8 animals per group. After refeeding, blood samples of the FRW group were assayed for AG in 7 mice only because of insufficient blood volume extracted at sacrifice for one animal. Data are presented as mean ± SEM plus individual values and were analyzed using three-way ANOVA (A), two-way ANOVA (B–D) or RM ANOVA (E–G) as described in Table S1. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0,0001. AG: Acyl-ghrelin. LEAP-2: Liver-expressing Antimicrobial Peptide.