REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-c-Fos	Cell Signaling Technology	Cat#2250S; RRID: AB_2247211
Goat polyclonal anti-CGRP	Abcam	Cat#ab36001; RRID: AB_725807
Mouse monoclonal anti-Annexin-A1	Santa Cruz Biotechnology	Cat#sc-12740; RRID: AB_2057007
Rabbit monoclonal anti-Iba1	Abcam	Cat#ab178846; RRID: AB_2636859
Rabbit monoclonal anti-NeuN	Abcam	Cat#ab177487; RRID: AB_2532109
Rabbit monoclonal anti-GFAP	Abcam	Cat#ab68428; RRID: AB_1209224
Donkey anti-rabbit IgG H&L (Alexa Fluor® 488) preadsorbed	Abcam	Cat#ab150061; RRID: AB_2571722
Goat anti-rabbit IgG H&L (Alexa Fluor® 488) preadsorbed	Abcam	Cat#ab150081; RRID: AB_2734747
Donkey anti-goat IgG H&L (Alexa Fluor® 594) preadsorbed	Abcam	Cat#ab150136; RRID: AB_2782994
Goat anti-mouse IgG H&L (Alexa Fluor® 594) preadsorbed	Abcam	Cat#ab150120; RRID: AB_2631447
Rabbit monoclonal anti-Annexin-A1	Abcam	Cat#ab214486; RRID: AB_2890907
Mouse monoclonal anti-α-tubulin	Beyotime Biotechnology	Cat#AF2827
HRP-labeled goat anti-mouse IgG (H + L)	Beyotime Biotechnology	Cat#A0216
HRP-labeled goat anti-rabbit IgG (H + L)	Beyotime Biotechnology	Cat#A0208
Chemicals, peptides, and recombinant proteins
Glyceryl trinitrate	Beijing Yimin Pharmaceutical Co., Ltd.	H11020289; CAS: 55-63-0
Rizatriptan benzoate	Sichuan zitonggong pharmaceutical Co., Ltd.	H20060352; CAS: 145202-66-0
2,2,2-tribromoethanol	Sigma-Aldrich	Cat#T48402; CAS: 75-80-9
2-Methyl-2-butanol	Sigma-Aldrich	Cat#152463; CAS: 75-85-4
TRIzol	Invitrogen	Cat#15596018; CAS: 9048-46-8
Ac2-26 TFA	MedChemExpress	Cat#HY-P1098A
Boc-MLF TFA	MedChemExpress	Cat#HY-103473A
Evans Blue	Sigma-Aldrich	Cat#E2129; CAS: 314-13-6
Critical commercial assays
O.C.T. TissueTek Compound	Sakura Finetek USA, Inc.	Cat#4583
Mounting medium with DAPI	Abcam	Cat#ab104139
NEBNext® Ultra™ RNA Library prep Kit for Illumina®	New England Biolabs	Cat#E7770
USER Enzyme	New England Biolabs	Cat#M5505L
Phusion High-Fidelity DNA polymerase	New England Biolabs	Cat#M0530L
RNAprep FastPure Tissue &Cell Kit	TSINGKE	Cat#TSP413
Goldenstar RT6 cDNA Synthesis Kit Ver.2	TSINGKE	Cat#TSK302S
2×T5 Fast qPCR Mix (SYBR Green I)	TSINGKE	Cat#TSE202
RIPA lysis buffer	Beyotime Biotechnology	Cat# P0013B
Phenylmethylsulfonyl fluoride	Beyotime Biotechnology	Cat#ST506
Protease inhibitor	Roche	Cat#05892970001
BCA protein Assay Kit	Beyotime Biotechnology	Cat#P0010S
5× SDS-PAGE loading buffer	Beyotime Biotechnology	Cat#P0015L
Super ECL plus western blotting substrate	Wansheng Haotian Biotechnology Co., Ltd	Cat#EZWB01
Deposited data
Raw RNA-seq data	This paper	SRA: PRJNA979279
Experimental models: Organisms/strains
C57BL/6J mice	SPF (Beijing) Biotechnology Co., Ltd.	RRID: MGI: 2160531
Software and algorithms
Algorithm for mechanical withdrawal thresholds	Christensen et al.57	https://bioapps.shinyapps.io/von_frey_app/
ImageJ	NIH	https://imagej.net/software/fiji/
HTSeq (v 0.13.5)	Anders et al.58	https://pypi.org/project/HTSeq/0.13.5/#files
DESeq R package (v 1.12.1)	Anders et al.59	https://www.bioconductor.org/packages//2.12/bioc/html/DESeq.html
OmicStudio tools	the Lianchuan Bioinformatics Cloud Platform	https://www.omicstudio.cn/tool
Graphpad prism 8	Graphpad	https://www.graphpad.com/dL/1057948/D599DCF7/
Adobe Illustrator 2022	Adobe	https://www.adobe.com
Other
Calibrated von Frey monofilaments	Danmic Global	Cat#514000-20C
Freezing microtome	Leica Biosystems	Cat#CM1950
Confocal microscope	Olympus	Cat#FV1000
Illumina Novaseq 6000 platform	Illumina	N/A
Real-Time PCR System	Bioer Technology	Cat#FQD-96A
Homogenizer workcenter	IKA	Cat#0003737025
Polyvinylidene difluoride membranes	Millipore	Cat# IPVH00010
Tanon 5200 chemiluminescent imaging system	Tanon Science & Technology	N/A
Stereotaxic divice	RWD Life Science	Cat#69105
Guide cannula	RWD Life Science	Cat#62003
Injection cannula	RWD Life Science	Cat#62203
Microliter syringe	Hamilton	Cat#1701
Syringe pump	KD Scientific	Cat#788130