TABLE 2.

Biofilm and effluent organism titers in mixed Sorbarod biofilm cultures of S. pneumoniae serotype 19 and M. catarrhalis ATCC 25238

Inoculum ratio (pneumococcus/ moraxella)	Pneumococcus titer		Moraxella titer
	Biofilm	Effluent	Biofilm	Effluent
100:100	13.74	9.47	12.99	8.60
100:10−1	13.77	9.69	13.12	8.47
100:10−2	13.75	9.77	13.23	8.44
100:10−4	13.54	9.34	13.60	8.43
Pneumococcus control	13.77	9.47
100:100	13.74	8.78	13.80	10.69
10−1:100	13.72	8.94	13.77	9.84
10−4:100	13.59	8.68	13.78	9.85
Moraxella control			13.92	9.84

^aAn undiluted broth culture of one organism was mixed with an equal volume of a dilution of the other organism and this mixture was used to inoculate a biofilm. After 24 h effluent was collected, the biofilm was harvested, and the organism titers were determined on selective media as described in the text. Control biofilms were inoculated with only one organism. Titers in biofilm (log₁₀) are expressed as recoverable CFU per filter; titers in effluent are expressed as CFU per milliliter.