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MitoView Green Mixture
| Reagent - stock solution concentration | Final concentration | Volume | Dilution |
|---|---|---|---|
| MitoView Green - 200 μM | 50 nM | 1 μL | 1:4000 |
| 1× Perm Buffer | N/A | 3999 μL | NA |
| Total | 4 mL | NA | |
The required volume of the mix is 100 μL per tube. For one sample you would have to make the redox markers staining times 2 (fully stained untreated sample, fully stained treated sample). Here we prepared 4 mL of Mix because of the high dilution factor of the stock solution.
