Table 6.

Primary SOD1 Antibody Mixture

Marker and Clone	Fluorophore	Final dilution	Volume (for 100 μL cell suspension)
SOD1/Cu-Zn SOD Antibody	N/A	1:25	4 μL
1× Perm Buffer	N/A	N/A	96 μL
Total			100 μL

The required volume of the mix is 100 μL per tube. The final volume has to be multiplied by the number of tubes. For one sample you would have to make the redox markers staining times 2 (untreated sample full staining, treated sample full staining). In case of a higher amount of samples, it is suggested to prepare a master mix with at least 10% extra volume (e.g., if 10 tubes are required, calculate on the basis of 11), so that even the last tube is filled properly avoiding the effect of sample loss when dosing.