Table 7.

Secondary Antibody Mixture Targeting SOD1

Marker and Clone	Fluorophore	Final dilution	Volume (for 100 μL cell suspension)
Goat anti-Rabbit IgG (H + L) Secondary Antibody,	DyLight 405	1:1000	1 μL
1× Perm Buffer	N/A	N/A	999 μL
Total			1 mL

The required volume of the mix is 100 μL per tube. For one sample you would have to make the redox markers staining times 2 (untreated sample full staining, treated sample full staining). Here we prepared 1 mL of Mix, enough for 9 tubes with 10% of extra volume.