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. 2023 Oct 14;4(4):102632. doi: 10.1016/j.xpro.2023.102632

Table 7.

Secondary Antibody Mixture Targeting SOD1

Marker and Clone Fluorophore Final dilution Volume (for 100 μL cell suspension)
Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLight 405 1:1000 1 μL
1× Perm Buffer N/A N/A 999 μL
Total 1 mL

The required volume of the mix is 100 μL per tube. For one sample you would have to make the redox markers staining times 2 (untreated sample full staining, treated sample full staining). Here we prepared 1 mL of Mix, enough for 9 tubes with 10% of extra volume.