Table 7.
Secondary Antibody Mixture Targeting SOD1
Marker and Clone | Fluorophore | Final dilution | Volume (for 100 μL cell suspension) |
---|---|---|---|
Goat anti-Rabbit IgG (H + L) Secondary Antibody, | DyLight 405 | 1:1000 | 1 μL |
1× Perm Buffer | N/A | N/A | 999 μL |
Total | 1 mL |
The required volume of the mix is 100 μL per tube. For one sample you would have to make the redox markers staining times 2 (untreated sample full staining, treated sample full staining). Here we prepared 1 mL of Mix, enough for 9 tubes with 10% of extra volume.