Complete RPMI 1640 medium

Reagent	Final concentration	Amount
RPMI	N/A	445 mL
FBS	10%	50 mL
Penicillin-Streptomycin (10,000 U/mL)	100 U/mL	5 mL
Total		500 mL

Medium is filtered (0.22 μm) and stored at 4°C up to 1 month.