FIG. 1.

(A) Restriction map of plasmid pPMT1 from P. multocida and organization of the integrated transposon Tn5706. Cleavage sites for restriction enzymes are abbreviated as follows: B, BclI; C, ClaI; EV, EcoRV; Ha, HaeIII; H, HindIII; P, PvuII. A distance scale (in kilobase pairs) is displayed below the map of pPMT1; the numbers in Tn5706 refer to the sequence deposited in the database (Y15510). The hatched boxes at the ends of IS1596 and IS1597 indicate the 18-bp inverted repeats, and the stacked brick-like boxes in IS1596 and IS1597 mark the extent and direction of transcription of the putative transposase reading frames. (B) Nucleotide sequence of IS1597. The deduced amino acid sequences of the 70- and 228-aa proteins are displayed in the one-letter code. Asterisks indicate translational stop codons. The 18-bp inverted repeated sequences are shown in boldface type. The internal directly repeated 34-bp sequence is displayed in lowercase letters. Important restriction endonuclease cleavage sites with regard to panel A are indicated. The numbers refer to the Tn5706 sequence deposited in the database.