Fig. 5.
BP-Btz enhances fracture repair in aged mice. (A) Eighteen-month-old WT mice received open tibial fracture surgery in both legs and were treated with Veh, BP, Btz, or BP-Btz on days 1, 3, and 5 post-fracture. Mice were euthanized 2 weeks or 4 weeks later and tibias were analyzed. (B) Callus volume by micro-CT. (C) Representative ABH-stained sections and histomorphometric analysis of woven bone and cartilage areas in the fracture calluses. Scale bar = 1 mm. Data were collected at 2 weeks post-treatment. n = 6–8 mice/group. One-way ANOVA with Dunnett’s test. *p < .05. (D) Bone strength by biomechanical testing. Data were collected at 4 weeks post-treatment. n = 3–5 mice (=6–10 legs)/group. One-way ANOVA with Dunnett’s test. *p < .05. All data are means + SD.