Figure 3.

IL-8 mediates NET release via ROS production. (A) Representative immunofluorescence images of DAPI-labeled DNA and immunolabeled H3Cit and MPO of neutrophils incubated with IL-8 (50 ng/mL) or control in the absence or presence of the ROS inhibitor DPI (10 µM). Scale bars, 25 µm. The measurements of (B) ROS levels and (C) extracellular DNA indicated the presence of NETs after the above treatment (n = 3 per group). (D) Western blot for H3Cit, NOX2, and NOX4 expression in neutrophils treated as mentioned elsewhere in this article (n = 3 per group). The protein samples were quantified using densitometry through ImageJ software and normalized. The data were expressed as mean ± SD and analyzed using a one-way ANOVA test. *P < 0.05, **P < 0.01.