TABLE 2.

General overview of growth factors in the culture medium of (A) prostate, gastric, spleen, cervical, endometrial and (B) ovarian cancers.

Types of cancer	Prostate	Gastric	Pancreatic	Cervical	Endometrial
(A)
References	Cheaito et al. 48	Yan et al. 46	Loomans et al. 47	Lõhmussaar et al. 57	Boretto et al. 144
Media composition	Advanced DMEM/F12	Advanced DMEM / F12	Advanced DMEM / F12	Advanced DMEM / F12	Advanced DMEM/F12 with L‐glutamine
	HEPES	HEPES		HEPES	HEPES
	Penicillin–Streptomycin	Penicillin–Streptomycin	Penicillin–Streptomycin	Penicillin–Streptomycin	Penicillin–Streptomycin
	Glutamax	WNT3A	B27	Glutamax	Glutamax
	Gentamicin/Amphotericin B	R‐spondin 1	EGF	B27	B27
	Plasmocin	Noggin	N‐acetylcysteine	Nicotinamide	Nicotinamide
	B27	B27	R‐spondin 1	EGF	EGF
	Nicotinamide	N‐acetylcysteine	Gastrin	FGF10	bFGF
	EGF	FGF10		A83‐01	FGF‐10
	FGF2	EGF		N‐acetylcysteine	A83‐01
	FGF10	Gastrin		Noggin	N‐acetylcysteine
	A83‐01	A83‐01		R‐spondin 1	Noggin
	N‐acetylcysteine	Y‐27632		p38 inhibitor	R‐spondin 1
	Noggin			Y‐27632	Insulin Transferrin Selenium
	R‐spondin 1			FGF7	Nitrogen
	p38 inhibitor			Forskolin	p38 inhibitor
	Dihydrotestosterone			CHIR	17‐β Estradiol
	Y‐27632			WNT surrogate	Y‐27632
				17‐β Estradiol

Types of cancer	Ovarian	Ovarian	Ovarian	Ovarian	Ovarian	Ovarian
(B)
References	Hill et al. 49	Kopper et al. 8	Maru et al. 54	Hoffmann et al. 52	Maenhoudt et al. 145	Nanki et al. 55
	Wan et al. 9	de Witte et al. 51
	Our group
Media composition	Advanced DMEM/F12	Advanced DMEM/F12	Advanced DMEM/F12	Advanced DMEM/F12	Advanced DMEM/F12	Advanced DMEM/F12
	HEPES	HEPES	HEPES	HEPES	HEPES	HEPES
	Penicillin–Streptomycin	Penicillin–Streptomycin	Penicillin–Streptomycin	Penicillin–Streptomycin	Penicillin–Streptomycin	Penicillin–Streptomycin
	Glutamax	Glutamax	Glutamax	Glutamax	Glutamax	Glutamax
	B27	Primocin	EGF	B27	B27	B27
	Nicotinamide	B27	Noggin	Nitrogen	Nitrogen	A83‐01
	EGF	Nicotinamide	R‐spondin 1	Nicotinamide	Nicotinamide	N‐acetylcysteine
	FGF2	EGF	Y‐27632	EGF	EGF	Noggin
	FGF10	FGF10		p38 inhibitor	HGF	WNT3A
	A83‐01	A83‐01		Y‐27632	A83‐01	R‐spondin 1
	N‐acetylcysteine	FGF10		BMP2	N‐acetylcysteine	Y‐27632
	Noggin	A83‐01			Noggin	IGF1
	R‐spondin 1	WNT3A			R‐spondin 1	Leu15‐Gastrin I
	p38 inhibitor	R‐spondin 1			p38 inhibitor
	Hydrocortisone	17‐β Estradiol			17‐β Estradiol
	Y‐27632	Hydrocortisone			Y‐27632
		Y‐27632			IGF1
					NRG1

Abbreviations: BMP2, bone morphogenetic protein 2; DMEM/12, Dulbecco's modified Eagle medium/nutrient mixture F12; ECF, epidermal growth factor; FGF2, fibroblast growth factor‐2; FGF10, fibroblast growth factor‐10; HGF, hepatocyte growth factor; HEPES, 4‐(2‐hydroxyethyl)‐1‐piperazine‐ethane‐sulfonic acid; IGF1, insulin‐like growth factor 1; NRG1, neuregulin‐1.