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. 2023 Oct 4;12(19):19904–19920. doi: 10.1002/cam4.6607

FIGURE 2.

FIGURE 2

LAP induced cell senescence in HER2+ breast cancer cells. (A, B) Cell senescence development and morphology alteration in SK‐S cells, compared to parental SK cells. SK cells were treated with LAP at 250 nM for 7 days and subjected to SA‐β‐Gal staining. (C, D) Cell cycle distribution of SK and SK‐S cells determined by flow cytometry. (E) Expression of DNA damage foci in SK and SK‐S cells detected by immunofluorescence. γH2AX (green), 53BP1 (red), and nuclear DNA (blue) counterstained with DAPI (scale bar: 10 μm). Magnification: ×630. (F, G) Gene expression of senescence markers in SK and SK‐S cells measured by Real‐time PCR. SK, parental SKBR3 cells; SK‐S, senescent SKBR3 cells. *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001. Data are expressed as mean ± SD (n = 3).