TABLE 1.

Sequence changes of and MICs for selected S. aureus isolates

Strain	Amino acid/nucleotidesa				MIC (μg/ml) of antibioticb
	gyrA codon		grlA codon		CPFX	TRFX	SPFX	LVFX
	84	88	80	84
Ciprofloxacin susceptible, trovafloxacin susceptible
Wild typec	S/TCA	E/GAA	S/TCC	E/GAA	0.25	0.03	0.06	0.19
SA74	S/TCA	E/GAA	S/TCC	E/GAA	0.25	0.023	0.06	0.125
Ciprofloxacin resistant, trovafloxacin susceptible
SA75	L/TTA	E/GAA	F/TTC	E/GAA	8	0.75	8	12
SA85	L/TTA	E/GAA	Y/TAC	E/GAA	64	0.75	8	8
Ciprofloxacin resistant, trovafloxacin resistant
SA198	L/TTA	E/GAA	F/TTC	K/AAA	128	8	16	32
SA22	L/TTA	E/GAA	Y/TAC	G/GGA	128	8	8	32
SA32	L/TTA	E/GAA	Y/TAC	G/GGA	128	16	8	32
SA92	L/TTA	E/GAA	Y/TAC	G/GGA	128	8	16	32

^aS. aureus numbering used throughout; mutations are in boldface type. 

^bMICs are the average of duplicate determinations; the duplicates did not differ by more than one twofold dilution. Antibiotic abbreviations: CPFX, ciprofloxacin; TRFX, trovafloxacin; SPFX, sparfloxacin; LVFX, levofloxacin. 

^cThe wild-type sequence is from references 8 and 11; wild-type MICs were determined on a derivative of antibiotic-sensitive standard strain 8325 (13).