Table 3.
Calculated IC50 values of fractions on α-amylase, lipase and α-glucosidase activities
| Alstonia boonei fractions | α-amylase | Pancreatic lipase | α-glucosidase | |||
|---|---|---|---|---|---|---|
| Inhibition (%) | IC50 (μg/mL) | Inhibition (%) | IC50 (μg/mL) | Inhibition (%) | IC50 (μg/mL) | |
| ME | 40.37 ± 2.43 | - | 41.23 ± 1.85 | - | 32.56 ± 5.28 | - |
| CFF | 44.64 ± 4.32 | - | 37.98 ± 1.53 | - | 45.92 ± 5.06 | - |
| CAF | 74.54 ± 1.40 | 1203 ± 0.364 | 65.42 ± 2.19 | 2.77 ± 0.063 | 42.49 ± 1.63 | - |
| CSF | 74.97 ± 3.19 | 206 ± 0.103 | 60.21 ± 4.16 | 25.37 ± 0.062 | 77.95 ± 4.29 | 11.97 ± 0.081 |
| Orlistat | NA | NA | 93.18 ± 2.97 | 0.068 ± 0.063 | NA | NA |
| Acarbose | 83.18 ± 2.97 | 64.58 ± 0.071 | NA | NA | 76.70 ± 2.63 | 2.84 ± 0.087 |
Alstonia boonei fractions: ME methanol extract, CAF crude alkaloid fraction, CSF crude saponin fraction. Results of inhibition (%) are demonstrated as Mean ± SD of 3-replicates of every fraction. IC50 values are mean ± SEM; (–) fractions/extract did not inhibit 50% of enzyme activity at 1000 μg/mL; NA not applicable