Table 2.
Study design and actions performed to followed piglets born to treated or non-treated sows
| Production phase | Day (D) | Action performed | Analysis | Non-treated sow | Treated-sow | |||
|---|---|---|---|---|---|---|---|---|
| Control (N = 10) | TP (N = 9) | IP (N = 12) | TS (N = 11) | TS-IP (N = 12) | ||||
| Lactation | D0 | Piglet treatment | 0 | 115 | 0 | 0 | 0 | |
| Piglet inoculation | 0 | 0 | 175 | 0 | 154 | |||
| Weight | 118 | 115 | 175 | 129 | 154 | |||
| Nasal sampling | M. hyorhinis, G. parasuis and S. suis PCRsa | 0 | 10 | 0 | 10 | 0 | ||
| Microbiota 16S sequencing | 0 | 5 | 0 | 5 | 0 | |||
| D7 | Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | |
| M. hyorhinis, G. parasuis and S. suis PCRsc | 50 | 45 | 60 | 55 | 60 | |||
| Microbiota 16S sequencing | 5 | 5 | 5 | 5 | 5 | |||
| D15 | Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | |
| M. hyorhinis, G. parasuis and S. suis PCRsc | 50 | 45 | 60 | 55 | 60 | |||
| Nursery | D21 | Weight | 101 | 85 | 118 | 89 | 97 | |
| Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | ||
| M. hyorhinis, G. parasuis and S. suis PCRsc | 50 | 45 | 60 | 55 | 60 | |||
| Microbiota 16S sequencing | 5 | 5 | 5 | 5 | 5 | |||
| WGS metagenomics | 5 | 5 | 5 | 5 | 5 | |||
| D49 | Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | |
| M. hyorhinis, G. parasuis and S. suis PCRsc | 25 | 20 | 25 | 25 | 25 | |||
| Microbiota 16S sequencing | 5 | 5 | 5 | 5 | 5 | |||
| WGS metagenomics | 5 | 5 | 5 | 5 | 5 | |||
a5 animals were randomly selected among the 10 samples for PCR testing
bCorresponded to one piglet per litter
cCorresponded to five piglet per litter
Control, non-treated piglets born to non-treated sows; TP, piglets treated with ceftiofur born to non-treated sows; IP, inoculated piglets born to non-treated sows; TS, piglets born to treated sows; TS-IP, inoculated piglets born to treated sows