Figure 1.

CD226 and TIGIT expression levels in Tregs and ex-Tregs. Islet-infiltrating cells from prediabetic 12-week-old female Treg-fate tracking NOD mice were assessed through spectral flow cytometry. Half-offset histograms (A) and quantification (B) of CD226 gMFI on GFP⁺YFP⁻ early Treg, GFP⁺YFP⁺CD44⁻CD62L⁺ naive stable Treg, GFP⁺YFP⁺CD44⁺CD62L⁻ E/M stable Treg, and GFP⁻YFP⁺ ex-Treg subsets. Fluorescence minus one (FMO) negative-staining controls display CD4⁺ T cells. Half-offset histograms (C) and quantification (D) of TIGIT gMFI on subsets as defined in B. Lines indicate the mean and 95% CI. P values are displayed and were determined from one-way ANOVA with the Bonferroni multiple comparisons test (n = 18).