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. 2023 Jun 22;72(11):1560–1573. doi: 10.2337/db22-1027

Figure 3.

Figure 3

FABP4 and AdEV protein secretion from adipocytes is decreased in p53-null mice. A: ELISA was used to measure FABP4 levels in 8-week-old, male, wild-type (WT) or p53-null mice serum. Serum from p53-null mice (obtained at 6 p.m. during the light phase) was evaluated for FABP4 levels. B: AdEVs were pelleted, purified from an equal volume of mouse serum from 8-week-old, male, WT or p53-null mice and resuspended in 1 mL PBS for analyses. AdEV size and amounts were measured by Nanosight analysis (see experiment details in Research Design and Methods). All results represent the mean ± SEM. **P < 0.01, ***P < 0.001. EV, extracellular vesicle.