FIGURE 2.

UV‐induced crosslinking of Calmodulin to CaMKII^L308BzF is Ca²⁺‐dependent. (a) Schematic representation of the experiment done in (b). (b) Coomassie‐stained SDS‐polyacrylamide gel showing absence of UV‐induced crosslinking of Calmodulin to CaMKII^WT (first 6 lanes), and dependence of UV‐induced crosslinking of Calmodulin to CaMKII^L308BzF on Ca²⁺ (second 6 lanes). CaM—Calmodulin. (c) Coomassie‐stained SDS‐polyacrylamide gel showing UV‐induced CaMKII dimer formation when 1 mM TCEP is used in the CaMKII buffer (lanes 1, 2, 5, and 6) and absence of this band when the CaMKII buffer is supplemented with 50 mM TCEP in the case of CaMKII^WT (lanes 3 and 4), but not in the case of CaMKII^L308BzF (lane 8). His‐tagged Calmodulin (His‐CaM) is present in lanes 5 and 6.