Table 1. Summary and Checklist of Parameters to Be Reported on In Vitro PPTT Experiments^a.

parameter	representative units	information
material characterization
method of purification		dialysis, ultracentrifugation...
conjugation-stabilizer
laser wavelength	nm
nanoparticle LSPR	nm
nanoparticle type and ligand information		MIRIBEL guidelinesb
biological characterization
cell culture details		MIRIBEL guidelinesb
cell type and origin		epithelial, human, cancerous...
appropriate controls
experimental protocol
nanomaterial quantity	ng/mL, μg/μL	amount of material in the nanoparticle samples
time of incubation	min, h
nanoparticle removal prior to irradiation	yes, no
irradiation dimension		2D, 3D, organoid, suspension...
type of well plate		flat bottom, U-bottom, cuvette...
time between administration and therapy	min, h
irradiation protocol
temperature
initial temperature	°C
final temperature	°C
temperature increase	°C	difference between initial and final temperature
temperature recording
type		IR thermal camera, probe...
time period		continuous, intermittent...
position		position regarding the sample (top, lateral, bottom...)
baseline and cool-off	s, min	temperature recorded before and after irradiation
laser
type		collimated, divergent
position	cm	position and distance to the sample (top, lateral, bottom...)
beam diameter/area	mm, mm2
power density	W/cm2
time of irradiation	s, min
after irradiation
method of assessment		techniques used to assess viability, toxicity...
time after treatment	min, h	time between the end of irradiation and viability assessment

^aThis table provides a summary of the parameters to be included on in vitro PPTT experiments. We provide representative units to use when reporting results.

^bMIRIBEL guidelines² complete the proposed table for characterization of bionano interactions.