Figure 1.

Experimental design and timeline. The experimental design for EOP and the subsequent treatment was illustrated in a timeline extending over 30 days. The mice were subjected to adaptive feeding for 7 days, after which they were randomly assigned to 10 groups based on body weight and total distance traveled. Each group comprised 10 mice. Following the assignment of groups, the mice were subjected to a daily, one-hour session on an elevated platform. They were orally administered with nutrients before being returned to their cages. Behavioral tests (OFT, NOR, EMP, LDB, and AT) were performed after 30 days of model administration to assess anxiety-relieving and cognitive improvement. Additionally, parameters such as body weight, food intake, coat state scores, and SPT were measured every week (as indicated by red dots in the figure). Finally, on day 42 of the experiment, the mice were humanely euthanized, and their brain tissues and serum were collected for analysis.