Isolation and Sequencing of Novel Vibrio Species

. Author manuscript; available in PMC: 2023 Oct 20.

Published in final edited form as: Cold Spring Harb Protoc. 2023 Jan 3;2023(1):22–34. doi: 10.1101/pdb.prot108067

Cycling step	Temperature	Time	No. of cycles
Initial denaturation	72°C	3 min	1×
Denaturation	95°C	30 sec	12×
Annealing	55°C	30 sec
Elongation	72 °C	30 sec
Final elongation	72°C	5 min	1×
Hold	10°C