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. Author manuscript; available in PMC: 2023 Oct 20.
Published in final edited form as: Cold Spring Harb Protoc. 2023 Jan 3;2023(1):22–34. doi: 10.1101/pdb.prot108067
Reagent Quantity for 10 mL
Orange G (Fisher Scientific AC416550100) 100 mg
Tris-EDTA solution (10×, pH 7.4; Fisher Scientific BP2477100) 5 mL
Glycerol (80% v/v) 5 mL

  1. Add Orange G to 1× Tris-EDTA solution (pH 7.4).

  2. Vortex to dissolve.

  3. Add 80% glycerol.

  4. Vortex to mix.

  5. Store at room temperature.

The buffer is stable for up to 5 yr at room temperature.