Table 2.
The mean ASFV genome copies per gram tissue (in log10) post-immunization.
| Lymph nodesa | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ton | Thy | Lun | Liv | Kid | Spl | Ileum | SLN | RPLN | TBLN | GHLN | RLN | ICLN | |
| Group Vb | |||||||||||||
| V1 | – | 2.55 | 4.50 | 4.37 | – | 3.95 | 0.70 | – | – | 2.60 | 3.96 | 1.50 | 0.88 |
| V2 | – | 0.74 | 2.20 | 2.46 | – | 1.54 | – | 2.41 | 3.50 | 5.09 | 3.22 | – | – |
| V3 | – | 0.71 | 3.70 | 3.50 | – | 3.55 | – | 3.71 | 3.08 | 4.17 | 3.91 | 2.91 | – |
| V4 | – | 2.64 | 2.60 | 0.83 | – | – | 1.45 | 2.23 | 2.93 | 4.15 | 2.36 | 4.22 | – |
| V5 | – | 1.65 | 2.20 | 1.59 | 1.64 | 3.47 | – | 2.64 | 3.68 | 3.44 | 2.30 | 1.53 | 3.38 |
| V6 | 0.71 | – | – | – | – | – | – | – | 2.18 | – | – | 1.42 | – |
| Group Wc | |||||||||||||
| W1 | 4.25 | 2.68 | 2.10 | 3.22 | – | 2.17 | – | 3.00 | 2.39 | 2.31 | – | – | 1.73 |
| W2 | – | – | – | 3.39 | – | – | – | – | – | 4.15 | – | – | – |
| W3 | – | – | – | – | – | – | – | – | – | – | – | – | – |
| W4 | 5.00 | 4.32 | 4.30 | 2.59 | – | 4.22 | 3.48 | 2.87 | 3.22 | 4.68 | 3.68 | 3.95 | 3.60 |
| W5 | 2.25 | – | 3.20 | 2.19 | 2.32 | 2.68 | 0.88 | 2.06 | 3.98 | 4.76 | 3.79 | 3.28 | 2.79 |
| W6 | – | 1.91 | – | – | 2.17 | – | – | – | – | – | – | 1.57 | – |
Ton: Tonsil; Thy: Thymus; Lun: Lungs; Liv: Liver; Kid: Kidney; Spl: Spleen.
a
Lymph node of different origins including SLN: submandibular; RPLN: retropharyngeal; TBLN: tracheobronchial; GHLN: gastrohepatic; RLN: renal; ICLN: ileocaecal. Samples were extracted in duplicates and qPCR were performed in duplicates – quadruplicates/ sample. –: not detected.
b
3 Group V pigs were culled at 7 dpi and the other 3 at 14 dpi.
c
Group W pigs were immunized at a high dose (106) and were culled at 17 dpi.